How to Improve Your Flow Cytometry Panel Design with More Accurate Spread Measurements: Webinar
BD Harmony Webinars by Bob Balderas, BD Life Sciences–Biosciences
Do you run multicolor flow cytometry experiments with more than 10 colors?
Do you use tools like Spillover Spread Matrix (SSM) to predict spread and design multicolor panels?
Do you find discrepancies between predicted and measured spread, resulting in sub-optimal resolution?
If the answer to these questions is yes, then this webinar is for you!
While designing multicolor flow cytometry panels, it is important to assess fluorescence spillover to prevent or minimize loss of resolution due to spreading. The SSM was developed as a tool to monitor and compare instrument performance over time, especially when experiments are standardized or calibrated across different instruments.1
The SSM is independent of fluorochrome brightness. While this feature is important for the comparison of instruments, it may lead to inaccurate spread prediction and sub-optimal panel design.
In this webinar, Bob Balderas, distinguished BD Biosciences fellow, VP biological sciences, VP market development, and Mirko Corselli, global scientific content manager, introduce a new, optimized method for more accurate spread assessment and explain how it can aid in panel design.
Watch the recorded webinar below.
