BD™ Cytometric Bead Array (CBA) Human IFN-α Flex Set

The BD CBA Human IFN-α Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IFN-α Flex Set will be 2,500 pg/mL. An example standard curve is shown in Figure 1.

The BD CBA Human IFN-α Flex Set cannot be used in the same assay well with the following BD CBA Human Soluble Protein Flex Set reagents:

Flex Set                                                Bead Position                Catalog Number

BD CBA Human IL-21 Flex Set                B8                                560358

BD CBA Human VEGF Flex Set                B8                                558336

The BD CBA Human IFN-α Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set or CD Marker Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets) nor with any BD CBA Human Soluble Receptor Protein Flex Set reagents. For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/cbasetup.

Performance

Limit of Detection: The theoretical limit of detection is 1.5 pg/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/mL, n=30) + 2 standard deviations.