Overview
BD immunoassay ELISA reagents contain antibody pairs that have each been developed to work together to quantitate soluble analytes in serum, plasma and tissue culture supernatants using sandwich ELISA.
The reagents:
- Come in three convenient sandwich ELISA formats and provide flexibility and cost efficiency for the analysis of tissue culture supernatants
- BD OptEIA™ Kits contain all necessary reagents and precoated 96-well plates for analysis
- BD OptEIA™ Sets include sufficient capture/detection reagents and standards for five or twenty 96-well plates
- Are composed of unlabeled capture antibodies, biotinylated detection antibodies and protein standards
- Offer a wide range of specificities and assay design flexibility with individual reagents as well as convenient set and kit formats
- Provide a cost-effective approach with single-vial reagents offering a wide range of specificities along with assay design flexibility to develop your own immunoassays
Features
RESOURCES AND TOOLS
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Frequently Asked Questions
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Protocols
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Tools
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Blocking the Plate
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Capture Antibody Preparation and Coating
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Getting Started
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Standard Preparation and Handling
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Standard and Sample Addition
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Stopping the Reaction and Reading the Plate
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TMB Substrate Preparation and Addition
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Troubleshooting: High Background
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Troubleshooting: Low/No Signal
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Troubleshooting: Poor Standard Curve
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Troubleshooting: Well-to-Well Precision
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Washing the Plates
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Working Detector Preparation
RELATED PRODUCTS
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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