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WTA Protocols
Cell Preparation and Cell Labeling
- This protocol describes preparation of single cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.
- Doc ID: 210964 Rev. 1.0
- Revision date: 07/2018
- The BD Single-Cell Multiplexing Kit (Cat. No. 633781) utilizes an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation. Every antibody-oligo in the kit, referred to as a Sample Tag, has a unique sample barcode conjugated to a human universal antibody. Up to 12 samples can be labelled and pooled prior to single cell capture with the BD Rhapsody™ Single-Cell Analysis system or other single cell analysis systems.
- Doc ID: 210970 Rev. 2.0
- Revision date: 02/2019
- This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides).
- Doc ID: 214394 Rev. 1.0
- Revision date: 07/2018
- This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(1 plex to 40 plex) with the BD® Single-Cell Multiplexing Kit.
- Doc ID: 214419 Rev. 2.0
- Revision date: 02/2019
- This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(41 plex to 100 plex) with the BD® Human Single-Cell Multiplexing Kit.
- Doc ID: 23-22354-00
- Revision date: 05/2020
cDNA Synthesis
- This protocol describes cell loading in the BD Rhapsody™ Cartridge and single cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
- Doc ID: 210967 Rev. 1.0
- Revision date: 07/2018
- This protocol describes cell loading in the BD Rhapsody™ Cartridge and single cell capture with the BD Rhapsody™ Single-Cell Analysis system with the BD Rhapsody™ Scanner.
- Doc ID: 210966 Rev. 1.0
- Revision date: 07/2018
Sequencing Library Preparation
- This protocol provides instructions on creating a single cell whole transcriptome mRNA library after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers.
- Doc ID: 23-21711-00
- Revision date: 07/2019
- This protocol provides instructions on creating single cell whole transcriptome mRNA and AbSeq protein detection libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers.
- Doc ID: 23-21751-00
- Revision date: 07/2019
- This protocol provides instructions on creating single cell whole transcriptome mRNA and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers.
- Doc ID: 23-21712-00
- Revision date: 07/2019
- This protocol provides instructions on creating single cell whole transcriptome mRNA, AbSeq, and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers.
- Doc ID: 23-21752-00
- Revision date: 07/2019
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