Why Choose BD Rhapsody™ ATAC-Seq?

The BD Rhapsody^™ HT Single-Cell Analysis System enables single-cell capture with reliable and reproducible results with minimal site-to-site variability making it suitable for translational, multi-center, multi-cohort studies. One readout can be used to analyze both protein and mRNA, reducing experimental time and increasing information derived from a single experiment. BD^® AbSeq Antibody-Oligonucleotide Conjugates allows high-parameter protein expression data simultaneously with single-cell RNA-Seq data to significantly improve your understanding of individual cells. For example, targeted panels like the BD Rhapsody^™ TCR/BCR Multiomic Assay Kit allow protein and whole transcriptome mRNA analysis to comprehensively understand immune cell function. Using genomic and proteomic information from single-cell analysis, you can concentrate on cells of interest for a deeper understanding. The BD FACSymphony^™ S6 Cell Sorter enables sorting of populations by using up to 50 parameters to define populations deep in your gating hierarchy. The detection technology in the BD FACSymphony^™ A5 SE Cell Analyzer has enhanced resolution across the visible spectrum to identify and analyze rare cell types. 

Learn more about the single-cell multiomics approach to comprehensively identify the gene and protein signature of T-cells.

The BD FACSymphony^™ Cell Analyzers leverage the benefits of high-parameter flow cytometry for simultaneous measurements, depending on the system, of up to 50 different characteristics of a single cell, accommodating your workflow needs. The BD FACSymphony^™ A5 SE Cell Analyzer offers flexibility with an optical design that enables both spectral unmixing and compensation workflows. Our broad array of flow cytometry reagents including BD Horizon Brilliant^™ Dyes, and BD Horizon RealYellow™ and BD Horizon RealBlue™ Fluorochromes with more than 9,000 human immunology research reagents can support cell therapy research. Made on demand, the BD OptiBuild^™ Reagents also provide lot-to-lot consistency that can be tailored to your requirements. At your disposal is a wide range of custom products including BD^® Abseq Reagents, instruments, conjugates, multicolor panels and cocktails to fit your needs.

BD products provide unique customizable end-to-end solutions with workflow flexibility for multiomic analysis and single-cell isolation of specific, rare cell populations. Led by you for your specific purpose, our team of scientists and experts can help with panel design, reagent selection, assay implementation, workflow optimization and experimental troubleshooting.

High-dimensional experiments result in more data to analyze, visualize and organize. Using the advanced computational tools within FlowJo^™ Software, the leading software for flow cytometry analysis,* or Data Analysis pipelines, a tool providing visualization and tertiary analysis for single-cell multiomics analysis, you can unlock the insights from your data, with accessible features across a wide breadth of research applications. FlowJo^™ Software enables flow cytometry data analysis with accessible features for immunophenotyping, cell cycle, proliferation, kinetics studies, quantitative population comparison, plate screening assays and more. Create publication worthy graphics using advanced algorithms through our plugin interface to analyze your high-dimensional data with the latest techniques to drive your research and discovery forward. 

BD^® Research Cloud, now available in the U.S., is a unique cloud-based ecosystem for the organization of and collaboration around flow cytometry experiments. The powerful software allows you to track experiments through customizable workflows, design new panels and create BD FACSDiva^™ Software-based experiments away from the cytometer enabling efficiency, visibility and collaboration for your discovery work. 

*In 2021, FlowJo^™ Software was cited in leading immunology peer-reviewed journals 80% of the time a flow cytometry analysis software package was cited.

Flow cytometry sample preparation can be automated using the BD FACSLyric^™ Flow Cytometer integrated with the BD FACSDuet^™ Premium Sample Preparation System. The sample preparation system is designed to complement and physically integrate with the flow cytometer to provide a complete end-to-end walkaway solution. This system helps streamline workflow and drive productivity while allowing for on-board automated antibody cocktail preparation which eliminates risk of errors due to manual pipetting. It supports different types of blood collection tubes and a wide variety of reagent vials delivering flexibility. The BD FACSLyric^™ Flow Cytometer enables efficiency and productivity with automated laser alignment and compensation stability over 60 days and allows flexibility for acquisition using a universal loader with 21 different loading options including multiwell plates. The distinctive assay portability feature allows easy and efficient transferability of assays across sites.

A study demonstrated that samples prepared with 20x BD Multitest™ 6-color TBNK Reagent + BD Trucount^™ Tubes on the automated BD FACSDuet^™ System Integrated with BD FACSLyric^™ Flow Cytometer vs. manual preparation led to:

The BD FACSLyric^™ Flow Cytometer has a unique assay portability feature that allows transfer of user-defined assays from one instrument to another, providing standardization and reproducible results across global manufacturing sites. This high-performance, highly sensitive flow cytometer demonstrates exceptional resolution and improved separation to make dim and rare populations easier to resolve. The BD FACSDuet^™ Premium Sample Preparation System provides accuracy and consistency in operation by minimizing manual intervention and an intuitive software interface minimizing training and variations due to different operators’ experience.  

Contract manufacturing of ready-to-use multicolor reagents panels in liquid, dried, or lyophilized formats are customized to your specifications and requirements. Dried and lyophilized reagents are manufactured using proprietary BD Horizon^™ Chroma Dried Panel technology, and have extended stability compared to liquid reagents. All-in-one tube format reduces the need for manual pipetting and cocktailing which improves consistency, reduces operator errors and day-to-day variability and saves time. Certain preformulated dried reagent cocktails are available, like BD Horizon^™ Dri TBNK + CD20 Panel  for cell therapy research workflows.

Our flagship analyzer, BD FACSLyric^™ Flow Cytometer is upgradeable to a 12-color configuration. It is powered by the BD FACSuite^™ Application used for acquisition and analysis that helps to support 21 CFR Part 11 compliance with password protection, electronic signatures, automatic record keeping and audit trails. We provide operational qualification (OQ) by a BD field service engineer for features related to 21 CFR Part 11 compliance for the BD FACSLyric^™ Flow Cytometer and the BD FACSuite™ Application. The BD FACSDuet^™ Premium Sample Preparation System allows traceability of samples and worklists through carrier barcoding and supports ISO 15189-accredited labs with compliance with complete workflow traceability.

With you in the driver’s seat and BD as your single partner for flow cytometry reagents, instruments and services you will benefits from synergies in user workflows and support provided.  BD Technical Services offer installation qualification (IQ), operational qualification (OQ) and technical service support to minimize instrument downtime. BD offers qualification assistance to help customers meet current Good Manufacturing Practice (cGMP) and Good Laboratory Practice (cGLP) standards. Explore our contract manufacturing offering of multicolor panels in liquid, dried or lyophilized formats to minimize errors and time associated with manual cocktailing of reagents. The BD FACSLyric^™ Flow Cytometer has an install base of more than 3,000 units globally.

Flow cytometry sample preparation can be automated using the BD FACSLyric^™ Flow Cytometer integrated with the BD FACSDuet^™ Premium Sample Preparation System. The sample preparation system is designed to complement and physically integrate with the flow cytometer to provide a complete end-to-end walkaway solution. This system helps streamline workflow and drive productivity while allowing for on-board automated antibody cocktail preparation which eliminates risk of errors due to manual pipetting. It supports different types of blood collection tubes and a wide variety of reagent vials delivering flexibility. The BD FACSLyric^™ Flow Cytometer enables efficiency and productivity with automated laser alignment and compensation stability over 60 days and allows flexibility for acquisition using a universal loader with 21 different loading options including multiwell plates. The distinctive assay portability feature allows easy and efficient transferability of assays across sites.

A study demonstrated that samples prepared with 20x BD Multitest™ 6-color TBNK Reagent + BD Trucount^™ Tubes on the automated BD FACSDuet^™ System Integrated with BD FACSLyric^™ Flow Cytometer vs. manual preparation led to:

The BD FACSLyric^™ Flow Cytometer has a unique assay portability feature that allows transfer of user-defined assays from one instrument to another, providing standardization and reproducible results across global manufacturing sites. This high-performance, highly sensitive flow cytometer demonstrates exceptional resolution and improved separation to make dim and rare populations easier to resolve. The BD FACSDuet^™ Premium Sample Preparation System provides accuracy and consistency in operation by minimizing manual intervention and an intuitive software interface minimizing training and variations due to different operators’ experience.  

Contract manufacturing of ready-to-use multicolor reagents panels in liquid, dried, or lyophilized formats are customized to your specifications and requirements. Dried and lyophilized reagents are manufactured using proprietary BD Horizon^™ Chroma Dried Panel technology, and have extended stability compared to liquid reagents. All-in-one tube format reduces the need for manual pipetting and cocktailing which improves consistency, reduces operator errors and day-to-day variability and saves time. Certain preformulated dried reagent cocktails are available, like BD Horizon^™ Dri TBNK + CD20 Panel  for cell therapy research workflows.

Our flagship analyzer, BD FACSLyric^™ Flow Cytometer is upgradeable to a 12-color configuration. It is powered by the BD FACSuite^™ Application used for acquisition and analysis that helps to support 21 CFR Part 11 compliance with password protection, electronic signatures, automatic record keeping and audit trails. We provide operational qualification (OQ) by a BD field service engineer for features related to 21 CFR Part 11 compliance for the BD FACSLyric^™ Flow Cytometer and the BD FACSuite™ Application. The BD FACSDuet^™ Premium Sample Preparation System allows traceability of samples and worklists through carrier barcoding and supports ISO 15189-accredited labs with compliance with complete workflow traceability.

With you in the driver’s seat and BD as your single partner for flow cytometry reagents, instruments and services you will benefits from synergies in user workflows and support provided.  BD Technical Services offer installation qualification (IQ), operational qualification (OQ) and technical service support to minimize instrument downtime. BD offers qualification assistance to help customers meet current Good Manufacturing Practice (cGMP) and Good Laboratory Practice (cGLP) standards. Explore our contract manufacturing offering of multicolor panels in liquid, dried or lyophilized formats to minimize errors and time associated with manual cocktailing of reagents. The BD FACSLyric^™ Flow Cytometer has an install base of more than 3,000 units globally.