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In combination with the BD OneFlow™ LST, it is intended as an aid in the diagnosis of chronic lymphocytic leukemia (CLL) and other B cell chronic lymphoproliferative diseases.

 

 

Overview

The BD OneFlow™ B-CLPD T1 is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.1*

 

The BD OneFlow™ B-CLPD T1 is intended for flow cytometric immunophenotyping of B cells in peripheral blood and bone marrow as an aid in the diagnosis of chronic lymphocytic leukemia (CLL) and other B cell chronic lymphoproliferative diseases.

 

Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ Lymphoid Screening Tube (LST), BD OneFlow™ Plasma Cell Screening Tube (PCST), BD OneFlow™ Plasma Cell Disorders (PCD) Tube and BD OneFlow™ Acute Leukemia Orientation Tube (ALOT).

 
performance1

View video describing the BD OneFlow™ Solution on the BD FACSLyric™ Flow Cytometer.

FEATURES

The BD OneFlow™ B-CLPD T1:

  • Is a preconfigured single-use, ready-to-use 8-color reagent provided in a single tube
  • Is a classification tube that is used for specimens with B lineage populations needing further investigation in combination with the BD OneFlow™ Lymphoid Screening Tube (LST)
  • Is available in the 20 test/box size (4 pouches of 5 tubes each)
  • Allows for reagent visual identification with boxes, pouches and tubes color coded with a lighter blue color than the one identifying BD OneFlow™ LST. The blue color (dark and light) identifies the BD OneFlow™ B-cell Chronic Lymphoproliferative Disease Panel

 

 

 

The BD OneFlow™ Reagents increase efficiency by simplifying instrument standardization, reducing the time to prepare the instrument as well as the technical burden and training needs.2

For BD FACSLyric™ Flow Cytometer users:

  • BD® CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility through Universal Setup
  • BD® FC Beads (7-Color, 5-Color and 2-Color Kits) support consistency of results, eliminating the need for using cells for compensation
  • BD® CS&T Beads and BD® FC Beads kits necessary for instrument setup and compensation can be used for other CE-IVD assays on the BD FACSLyric™ Flow Cytometer

 

For BD FACSCanto™ II Flow Cytometer users:

  • BD FACSDiva™ CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility with Application Settings
  • BD OneFlow™ Setup Beads ensure data accuracy and reproducibility by providing assay-specific target values, as per EuroFlow™ SOPs.3
  • Specifically designed BD® FC Beads eliminate the need for using single-vial reagents and cells for compensation 

The BD OneFlow™ B-CLPD T1 reagent composition

 

AntibodyFluorochromeCloneTarget Populations
CD23FITCEBVCS-5Contributes to the classification of CLL or other B-cell chronic lymphoproliferative diseases

CD10

PEMEM-78Contributes to the classification of CLL or other B-cell chronic lymphoproliferative diseases
CD79bPerCP-Cy 5.5SN8Contributes to the classification of CLL or other B-cell chronic lymphoproliferative diseases
CD19PE-CY 7 SJ25-C1Backbone marker. In common with BD OneFlow™ LST
CD200APCMRC 0X-104Contributes to the classification of CLL or other B-cell chronic lymphoproliferative diseases
CD43APC-H71G10Contributes to the classification of CLL or other B-cell chronic lymphoproliferative diseases
CD20BD Horizon™ V450L27Backbone marker. In common with BD OneFlow™ LST
CD45BD Horizon™ V500-C2D1Backbone marker. In common with BD OneFlow™ LST

 

The EuroFlow antibody panel article1* has a full description of the utility of the antibodies chosen for the BD OneFlow™ B-CLPD T1.

 

 

APPLICATIONS

BD OneFlow™ B-CLPD T1 Lab Report (normal specimens) when used with the BD FACSLyric™ Flow Cytometer

Laboratory report of a normal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ B-CLPD T1 single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4.
BD OneFlow™ B-CLPD T1 Lab Report (abnormal specimens) when used with the BD FACSLyric™ Flow Cytometer

Laboratory report of an abnormal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ B-CLPD T1 single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4.
BD OneFlow™ Reagents can be run on both the BD FACSLyric™ and BD FACSCanto™ II Flow Cytometers with equivalent results.

Periodic and daily setup

 
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References

  1. van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120

  2. Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779

  3. Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122
For Research Use Only. Not for use in diagnostic or therapeutic procedures.