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Reagents
- Flow Cytometry Reagents
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蛋白质印迹试剂
- 免疫分析 试剂
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Single-Cell Multiomics Reagents
- 附件
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
- Dehydrated Culture Media
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- 附件
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
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Death Staurosporine
Induction of Apoptosis by Treatment with Staurosporine
Staurosporine ( Streptomyces staurospores) is a relatively non-selective protein kinase inhibitor, which blocks many kinases to different degrees. Staurosporine is often used as a general method for inducing apoptosis 1.
Protocol for Staurosporine-induced apoptosis:
- Add 1 µM (final concentration) staurosporine (eg, Sigma S6942) to cell suspension (e.g., 5 x 10 5 cells/ml in tissue culture medium).
- Perform a time course to obtain optimum results; a 1-6 hr incubation at 37°C is suggested. *Note: some cells or cell lines may take up to 12 hours or more to induce apoptosis.
- Proceed with assays designed to evaluate induction of apoptosis.
Kabir J, Lobo M, Zachary I. 2002 Staurosporine induces endothelial cell apoptosis via focal adhesion kinase dephosphorylation and focal adhesion disassembly independent of focal adhesion kinase proteolysis. J. Biochem. 367: 145-155.
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