Preparing Single Cell Suspensions

This protocol describes preparation of single cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody^™ Cartridge. This protocol does not describe tissue dissociation.
 

Co-staining cells with fluorescent antibodies and BD Oligo-Conjugated Antibodies

In this technical bulletin, we address how to co-stain cells with the same clone of both fluorescent antibodies and oligonucleotide-conjugated antibodies (BD^® Single-Cell Multiplexing Kit or BD^® AbSeq Ab-Oligos).
 

Single Cell Labelling with the BD^® Single-Cell Multiplexing Kits

The BD^® Human Single-Cell Multiplexing Kit (Cat. No. 633781) and the BD^® Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793) utilize an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation.
 

Single Cell Labeling with BD^® Flex Single-Cell Multiplexing Kits

BD^® Flex Single-Cell Multiplexing Kits utilize an innovative antibody-oligo technology to provide higher sample throughput for single-cell library preparation, as well as improve detection and elimination of multiplet data from single-cell experiments.

Single Cell Labelling with BD^® AbSeq Ab-Oligos (1 plex to 40 plex)

This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) for antigen-expression profiling with BD Rhapsody^™ single cell capture and downstream library preparation.

Single-Cell Labeling with BD^® AbSeq Ab-Oligos (41 plex to 100 plex)

This protocol describes the use of BD^® AbSeq Ab-Oligos (antibody-oligonucleotides) for antigen expression profiling with BD Rhapsody^™ single-cell capture and downstream library preparation.

dCODE Dextramer^® (RiO) staining protocol

This protocol provides instructions on cell labeling with dCODE Dextramer^® and optional BD AbSeq Ab-Oligos.
 

Single Cell Labelling with the BD^®  Single-Cell Multiplexing Kit and BD^® AbSeq Ab-Oligos (1 plex to 40 plex)

This protocol describes use of BD^® AbSeq Ab-Oligos (antibody-oligonucleotides) with the BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781) or BD^® Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793).
 

Single-Cell Labeling with BD^®  Single-Cell Multiplexing Kit and BD^® AbSeq Ab-Oligos (41 plex to 100 plex)

This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) (41 plex to 100 plex) with the BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781).

Single-Cell Labeling with BD^® AbSeq Ab-Oligos for Intracellular CITE-seq

This protocol details how to stain for intracellular antigens using BD^® Intracellular AbSeq (IC-AbSeq) antibodies for profiling with the BD Rhapsody^™ system.
 

Single-Cell Labeling with BD^® Flex Single-Cell Multiplexing Kits and BD^® AbSeq Ab-Oligos

This protocol describes the use of BD^® AbSeq Ab-Oligos (antibody-oligonucleotides) with the BD^® Flex Single-Cell Multiplexing Kits (Cat. no. 633849-633852).
 

BD^® AbSeq Immune Discovery Panel (IDP)

The IDP consists of 30 different specificities against major human immune markers combined in a single tube. Designed and optimized to work on the BD Rhapsody^™ Single-Cell Analysis System, the IDP is tested to work seamlessly with BD Rhapsody Single-Cell RNA Assays and Multiplexing Kits. In this brochure, we also describe steps for reconstituting the lyophilized IDP and staining cells.