Acquire your EV samples and explore the power of “OR” thresholding

Step 3: Apply proper controls, calibration and thresholding strategy
 

• Use MiFlowCyt-EV framework for publication to guide you on assay controls and SP SSC and fluorescence calibration
• Use “OR” thresholding for SP SSC and relevant fluorescent parameters for maximum information
• Consider running serial dilution series to check for absence of swarming

• An easy-to-use Solution
  With its single-tube format, the BD Multitest™ 6-Color TBNK reagent decreases cost by reducing the number of tubes and sample processing time. The reagent includes a cocktail of multiple fluorescently labeled monoclonal antibodies, premixed at the appropriate titer to ensure quality staining.
  
  
• Accurate cell counts with BD Trucount™ Tubes
  BD Trucount™ Tubes help to ensure more accurate cell counts - requires no additional pipetting steps, precise and reproducible. The tubes contain a lyophilized pellet of fluorescent beads that serves as an internal standard. BD FACSuite™  Clinical Application automatically calculates lymphocyte absolute counts and subset percentages.
  
  
• Improve safety, reduce hands-on time
  The BD Multitest™ 6-Color TBNK reagent employs a no-wash formula that reduces tube handling. Processing fewer tubes in less time may decrease potential exposure to biohazardous material, improving safety for laboratory personnel.
  
  

• Increase productivity
  

  Together with the BD FACSLyric™ cell analyzer and BD FACSuite™ software, the BD FACS™ Universal Loader option and the BD FACSDuet™ Sample Preparation System, the BD Multitest™ 6-Color TBNK reagent provides a fully automated, standardized and efficient workflow for lymphocyte enumeration to best suit your routine immunophenotyping needs.