Acquire your EV samples and explore the power of “OR” thresholding
Step 3: Apply proper controls, calibration and thresholding strategy
- Use MiFlowCyt-EV framework for publication to guide you on assay controls and SP SSC and fluorescence calibration
- Use “OR” thresholding for SP SSC and relevant fluorescent parameters for maximum information
- Consider running serial dilution series to check for absence of swarming
| 3.1 Buffy only* | 3.5 Single-stained controls* |
| 3.2 Buffer with reagents* | 3.6 Procedural controls* |
| 3.3 Unstained controls* | 3.7 Serial dilution* |
| 3.4 Isotype controls | 3.8 Detergent-treated EV samples |
Welsh JA, et al. Journal of Extracellular Vesicles. 20209;(1):1713526.
Overview
Flow cytometry is widely used for disease-specific assessments and immunophenotyping of immune cell populations. This evaluation help in diagnosis of immune deficiencies. Determining lymphocyte subsets can generate more insights of the immune system than a complete blood count (CBC).
BD Multitest™ 6-Color TBNK reagent with optional BD Trucount™ Tubes is a six-color direct immunofluorescence reagent for use in identifying and determining the percentages and absolute counts of T, B, and natural killer (NK) cells, as well as the CD4+ and CD8+ subpopulations of T cells, in peripheral blood on a BD flow cytometer.
Why BD Multitest™ 6-color
TBNK Kit?
- Performs lymphocyte enumeration of mature T, B and NK cells in a single tube
- Provides the option of absolute counts when paired with BD Trucount™ Tubes
- Works in a fully integrated system with BD FACSDuet™ Sample Preparation System for automated sample preparation allwoing a complete automated workflow from sample to answer, reducing hands-on time and error-prone steps.
- Can be used with BD FAC™ Universal Loader for automated sample acquisition on BD FACSLyric™
- CE-IVD assay modules with built-in templates and automated gating to work on BD FACSLyric™ instrument software for setup, QC, acquisition analysis and reporting. Seamless integration of data reporting of test and patient results through bidirectional LIS connectivity.
The BD Multitest™ 6-Color TBNK Kit Simplifies Your Workflow and Increases Productivity
- An easy-to-use Solution
With its single-tube format, the BD Multitest™ 6-Color TBNK reagent decreases cost by reducing the number of tubes and sample processing time. The reagent includes a cocktail of multiple fluorescently labeled monoclonal antibodies, premixed at the appropriate titer to ensure quality staining.
- Accurate cell counts with BD Trucount™ Tubes
BD Trucount™ Tubes help to ensure more accurate cell counts - requires no additional pipetting steps, precise and reproducible. The tubes contain a lyophilized pellet of fluorescent beads that serves as an internal standard. BD FACSuite™ Clinical Application automatically calculates lymphocyte absolute counts and subset percentages.
- Improve safety, reduce hands-on time
The BD Multitest™ 6-Color TBNK reagent employs a no-wash formula that reduces tube handling. Processing fewer tubes in less time may decrease potential exposure to biohazardous material, improving safety for laboratory personnel.
Increase productivity
Together with the BD FACSLyric™ cell analyzer and BD FACSuite™ software, the BD FACS™ Universal Loader option and the BD FACSDuet™ Sample Preparation System, the BD Multitest™ 6-Color TBNK reagent provides a fully automated, standardized and efficient workflow for lymphocyte enumeration to best suit your routine immunophenotyping needs.
Applications
The BD Multitest™ 6-Color TBNK reagent with Trucount™ tube for accurate and reliable enumeration of mature T, B and NK cells.
A hematological normal adult sample with BD Multitest™ 6 Color TBNK in a BD Trucount™ Tube wasacquired on a BD FACSLyric™ Flow Cytometer.
BD FACSLyric™ laboratory report showing data collected with BD Trucount™ Tubes. See the BD FACSLyric™ Clinical Reference System, which provides information on gating and troubleshooting.
*Certain restrictions apply and offers may vary in each country. Valid in participating countries only. Valid once per user only and on selected instruments. Not valid for distributor or third-party orders. Cannot be combined with any other offer. Valid until September 31, 2023. BD retains the right to cancel this offer at any time. Please contact your local sales representative for more information.
BD FACSLyric™ Flow Cytometer with the BD FACSuite™ Clinical and BD FACSuite™ applications is an in vitro diagnostic medical device bearing a CE mark.
BD FACSDiscover™ S8 Cell Sorter, an RUO product, has not been CE marked yet and cannot be made available until CE marking has been completed.
All other instruments displayed above are for Research Use Only. Not for use in diagnostic or therapeutic procedures.
BD Flow Cytometers are Class 1 Laser Products.
