Preparation And Storage
Recommended Assay Procedures
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The ER-MP23 monoclonal antibody specifically recognizes the extracellular domains of the mouse CD301a and CD301b homologs. CD301a is also known as macrophage galactose-type C-type lectin 1 (MGL1) which is encoded by Clec10a (C-type lectin domain family 10 member A). CD301b is likewise known as macrophage galactose-type C-type lectin 2 (MGL2) and is encoded by Mgl2 (Macrophage galactose N-acetyl-galactosamine specific lectin 2). Mouse CD301a and CD301b are ~42 kDa type II transmembrane glycoproteins that are each comprised of an extracellular region with a carbohydrate recognition domain (CRD) followed by a transmembrane region and a cytoplasmic tail. CD301a is expressed on a subset of macrophages, conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) whereas CD301b is expressed on cDCs and immature DCs. CD301a binds selectively to molecules that contain Lewis X or Lewis A structures. CD301b recognizes molecules having galactose and N-actetylgalactosamine (GalNAc) residues. Both receptors are involved in the recognition and endocytosis of glycoproteins and play roles in tissue remodeling, clearance of apoptotic cells, and defense against tumor cells. The ER-MP23 antibody can reportedly inhibit the binding of carbohydrate ligands to CD301a and CD301b.
Development References (6)
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Denda-Nagai K, Aida S, Saba K, et al. Distribution and function of macrophage galactose-type C-type lectin 2 (MGL2/CD301b): efficient uptake and presentation of glycosylated antigens by dendritic cells.. J Biol Chem. 2010; 285(25):19193-204. (Biology). View Reference
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Dupasquier M, Stoitzner P, Wan H, et al. The dermal microenvironment induces the expression of the alternative activation marker CD301/mMGL in mononuclear phagocytes, independent of IL-4/IL-13 signaling.. J Leukoc Biol. 2006; 80(4):838-49. (Clone-specific: Blocking, ELISA, Flow cytometry, Immunofluorescence). View Reference
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Dupasquier M, Stoitzner P, van Oudenaren A, Romani N, Leenen PJ. Macrophages and dendritic cells constitute a major subpopulation of cells in the mouse dermis.. J Invest Dermatol. 2004; 123(5):876-9. (Clone-specific: Immunofluorescence). View Reference
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Geutskens SB, Otonkoski T, Pulkkinen MA, Drexhage HA, Leenen PJ. Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.. J Leukoc Biol. 2005; 78(4):845-52. (Clone-specific: Immunofluorescence, Immunohistochemistry). View Reference
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Jansen A, Homo-Delarche F, Hooijkaas H, Leenen PJ, Dardenne M, Drexhage HA. Immunohistochemical characterization of monocytes-macrophages and dendritic cells involved in the initiation of the insulitis and beta-cell destruction in NOD mice.. Diabetes. 1994; 43(5):667-75. (Clone-specific: Immunohistochemistry). View Reference
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Leenen PJ, de Bruijn MF, Voerman JS, Campbell PA, van Ewijk W. Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods. 1994; 174(1-2):5-19. (Clone-specific). View Reference
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