Single-Cell Multiomics Protocols

Sample Preparation and Cell Labeling 

Preparing Single Cell Suspensions>

This protocol describes preparation of single cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.
  

Co-staining cells with fluorescent antibodies and BD Oligo-Conjugated Antibodies>

Here, we address how to co-stain cells with the same clone of both fluorescent antibodies and oligonucleotide-conjugated antibodies (BD® Single-Cell Multiplexing Kit or BD® AbSeq Ab-Oligos).
 

Single Cell Labelling with the BD Single-Cell Multiplexing Kit>

The BD Single-Cell Multiplexing Kit (Cat. No. 633781) utilizes an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation. Every antibody-oligo in the kit, referred to as a Sample Tag, has a unique sample barcode conjugated to a human universal antibody. Up to 12 samples can be labelled and pooled prior to single cell capture with the BD Rhapsody™ Single-Cell Analysis system or other single cell analysis systems.
 

Single Cell Labelling with BD^™ AbSeq Ab-Oligos>

This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides).

dCODE Dextramer^® (RiO) staining protocol>

This protocol provides instructions on cell labeling with dCODE Dextramer® and optional BD AbSeq Ab-Oligos..

Single Cell Labelling with the BD Single-Cell Multiplexing Kit and BD^® AbSeq Ab-Oligos (1 plex to 40 plex)>

This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) (1 plex to 40 plex) with the BD® Single-Cell Multiplexing Kit.
 

Single-Cell Labeling with BD Single-Cell Multiplexing Kit and BD^® AbSeq Ab-Oligos (41 plex to 100 plex)>

This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) (41 plex to 100 plex) with the BD® Human Single-Cell Multiplexing Kit.

BD^® AbSeq Immune Discovery Panel (IDP)>

The IDP consists of 30 different specificities against major human immune markers combined in a single tube. Designed and optimized to work on the BD Rhapsody™ Single-Cell Analysis System, the IDP is tested to work seamlessly with BD Rhapsody Single-Cell RNA Assays and Multiplexing Kits. In this brochure, we also describe steps for reconstituting the lyophilized IDP and staining cells