-
Reagents
- Flow Cytometry Reagents
-
Western Blotting and Molecular Reagents
-
Flow Cytometry Reagents
- Immunoassay Reagents
- Single Cell Multiomics Reagents
-
Cell Preparation
-
Functional Assays
-
Microscopy and Imaging Reagents
- Western Blotting And Molecular Reagents
- Cell Preparation Separation Reagents
- Functional Cell Based Reagents
- Microscopy Imaging Reagents
- Single Cell Multiomics Reagents
- Single Cell Multinomics Reagents
-
Protocols
- BSB Protocol
-
Setting Compensation Multicolor Flow
-
Tissues Section Stain
-
Immunomicroscopy
-
Immunohistochemical
-
Immunofluorescence
-
Frozen Tissue
-
Parafin Sections
-
Fix Perm Kits
-
Protocol Direct Immunofluorscence Staining
-
Uses of Fc Block
-
Stain Lyse Wash
-
Stain Lyse No Wash
-
Mouse Splenocytes
-
Mouse Rat Leukocytes
-
Isotype Control
-
Indirect Staining Mononuclear Cells
-
Immunopurification
-
Human PBMCs
-
Human Whole Blood Samples
-
Escapee Phenomenon
-
Agarose Conjugates
-
Anti Phosphotyrosine Biotin Conjugates
-
Soluble Antibodies
-
Rabbit Polyclonal Antibodies
-
Monocloncal Antibodies
-
Horseradish Peroxidase
-
Certified Reagents
-
Biotinylated Antibodies
-
Agarose Conjugates X712261
-
Surface Staining
-
Platelet Activation
-
Intracellular Staining
-
Indirect Immunofluorescence
-
Mouse Ige
-
Cytokine Elisa
-
Induction Fas
-
Induction Dx2
-
Apoptosis By Treatment Staurosporine
-
Cell Death
-
Apo Brdu
-
Apo Direct
-
Human Cyclins
-
Detection Ki 67
-
Brdu Detection
-
Targeted mRNA Protocols
-
WTA Protocols
-
360040667732 Protocols
-
360023293831 AbSeq Protocols
-
360039007471 VDJ CDR3 Protocols
-
Annexin V Staining Protocol
-
Western Blotting with Horseradish Peroxidase Conjugates or Alkaline Phosphatase Conjugates
-
Tissue Preparation for Surface Antigen Staining
-
Account Support
-
Account FAQs
- Account FAQ Answer 1
- Account FAQ Answer 2
- Account FAQ Answer 3
- Account FAQ Answer 4
- Account FAQ Answer 5
- Account FAQ Answer 6
- Account FAQ Answer 7
- Account FAQ Answer 8
- Account FAQ Answer 9
- Account FAQ Answer 10
- Account FAQ Answer 11
- Account FAQ Answer 12
- Account FAQ Answer 13
- Account FAQ Answer 14
- Account FAQ Answer 15
- Account FAQ Answer 16
- Account FAQ Answer 21
- Create Account
- Manage Account Settings
-
PrivacyPolicy
-
Terms and Conditions
-
Account FAQs
-
- Account FAQ Answer 1
- Account FAQ Answer 2
- Account FAQ Answer 3
- Account FAQ Answer 4
- Account FAQ Answer 5
- Account FAQ Answer 6
- Account FAQ Answer 7
- Account FAQ Answer 8
- Account FAQ Answer 9
- Account FAQ Answer 10
- Account FAQ Answer 11
- Account FAQ Answer 12
- Account FAQ Answer 13
- Account FAQ Answer 14
- Account FAQ Answer 15
- Account FAQ Answer 16
- Account FAQ Answer 21
- Korea (Korea)
- 국가 / 언어 변경
Old Browser
Flow cytometric analysis of CD152 expression on stimulated human peripheral mononuclear cells. Peripheral blood mononuclear cells were stimulated with concanavalin A for 3 days, then stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 555574; dashed line histogram) or PE Mouse Anti-Human CD152 (Cat. No. 555853/560939/557301; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable activated cells. Flow cytometry was performed on a BD FACScan™ system.
BD Pharmingen™ PE Mouse Anti-Human CD152
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
The BNI3 monoclonal antibody specifically binds to the human cytolytic T lymphocyte-associated antigen (CTLA-4), also known as CD152. CTLA-4 is transiently expressed on activated CD28+ T cells and binds to CD80 and CD86 present on antigen presenting cells (APC) with high avidity. This interaction appears to deliver a negative regulatory signal to the T cell. Recent reports indicate that CTLA-4 is also expressed on B cells when cultured with activated T cells, suggesting a role for CTLA-4 in the regulation of B-cell response. Immobilized BNI3 antibody enhances T-cell proliferation induced by antibody-mediated crosslinking of CD3 and CD28. Recent studies have shown that CD152 can be expressed by regulatory T (Treg) cells. After cellular fixation and permeabilization, the BNI3 antibody can stain intracellular CD152 expressed in T cells including Treg cells. Clone BNI3 was studied in the VI Leukocyte Typing Workshop.
Clone BNI3 also cross-reacts with a subset of peripheral blood lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys, following Concanavalin A (Con A) treatment. The distribution of BNI3+ cells following activation is similar to that observed with peripheral blood lymphocytes from normal human donors.
Development References (5)
-
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
-
Cabezon R, Sintes J, Llinas L, Benitez-Ribas D. Analysis of HLDA9 mAbs on plasmacytoid dendritic cell. Immunol Lett. 2011; 134(2):167-173. (Clone-specific: Flow cytometry). View Reference
-
Kuiper HM, Brouwer M, Linsley PS, van Lier RA. Activated T cells can induce high levels of CTLA-4 expression on B cells. J Immunol. 1995; 155(4):1776-1783. (Biology). View Reference
-
Lindsten T, Lee KP, Harris ES, et al. Characterization of CTLA-4 structure and expression on human T cells. J Immunol. 1993; 151(7):3489-3499. (Biology). View Reference
-
Morton PA, Fu XT, Stewart JA, et al. Differential effects of CTLA-4 substitutions on the binding of human CD80 (B7-1) and CD86 (B7-2). J Immunol. 1996; 156(3):1047-1054. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
23-22944-00
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.